Roads are important for human development, so in biodiverse tropical regions strong evidence on the impact on wildlife must be collected to support the implementation of measures to reduce it. Mesoamerica is a biodiverse region and road expansion and improving is on the way. We lack of proper data on the Mesoamerican regions of the implications of the impact of roads on wildlife and specific mitigation measures. We need to display with solid research what is beneath for proper mitigation measures. We start looking for a study area where we can compile evidence of road effect on wildlife. We were looking for an old primary road, an old protected area surrounding it, with vulnerable species, evidence of roadkills and lack of mitigation measures. Route 2: Interamericana Sur was in operation in 1946 and in 1945 was proposed the protection of a Quercus forest buffer of 2 km from each side of the road. On the surrounding protected areas we identified, endangered species like Tapir (Tapirus bairdii), Dice's cottontail rabbit (Sylvilagus dicei) (vulnerable, lack of information and endemic of this Mountain range) and Oncilla (Leopardus tigrinus oncilla), vulnerable and suspected to be a different species from South American population. From surveying this area, we can find evidence of road impact on wildlife from more than 70 years. We plan to survey 45 km of this road and the environmental fragile areas (2 national parks and 2 forest reserves) surrounding it to identified the impact on wildlife and recommend mitigation measures. Camera traps and road surveys will give us information about the distribution and vulnerability towards roads. We will Identified vertebrates crossings and roadkills; identified density, presence and distribution of mammals at the protected areas and propose mitigation measures to the government for their implementation. Our main preliminary results expected 4.000 roadkills for a year mostly birds, many endemic rabbits, one tapir and an oncilla. We have identified 191 drainages, most of them are blocked and 47 have the potential to be retrofitted as a wildlife crossing. From the cameras on 20 of the drainages, we found the use as a wildlife crossing by coyote, endemic rabbit, weasel and squirrel. In addition, there is no safe crossing yet for tapirs through drainages, there is just two big drainages but their entrance is very steep. We are working closely with the Ministry of Transportation and Environments because the proposal and implementation of measures for wildlife on existing roads is a brand new process.